Proteins with high purity for drug screening, SPR and crystallography
From pilot expression to scale-up production (10-15L)
His, MBP, Strep, GST, SUMO, Flag tag, etc.
AviTag to produce biotinylated protein
E. coli expression system
Baculovirus expression system (Sf9, High Five)
Mammalian cell expression system (Expi293, ExpiCHO)
Affinity chromatography
SEC (Gel filtration)
IEX (e.g. HiTrapQ HP)
Protein purity – SDS-PAGE, analytical HPLC
Protein homogeneity and aggregation states – DLS
Protein MW, modification, etc. – LC-MS
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