One-stop PROTAC service platform provides solutions in all stages of PROTAC-induced UPS-mediated target protein degradation, from cell permeability, binary target engagement, ternary complex formation, target ubiquitination, proteasomal recruitment, to target degradation
Monitor endogenous target protein degradation through large variety of cell-based assays (lytic and kinetic HiBiT, NanoLuc, Simple Western on JESS, In-cell ELISA, AlphaLISA, Western Blot, FCM)
Customized HiBiT and NanoLuc knock-in cell line generation that allows highly sensitive, high-throughput, quantitative bioluminescence measurement on endogenous target protein, either endpoint or kinetic
Multiplexing HiBiT assay and cell viability assay for accurate interpretation of target degradation data
Determination of ternary complex formation by TR-FRET, NanoBRET, and SPR gives strong support for PROTAC design and optimization.
Rank-order compounds by binding affinity using high-throughput HTRF, NanoBRET, and biophysics SPR assays
Tailored degradation phenotype assays
Discovery team with deep expertise and extensive experience in PROTAC evaluation
Timely troubleshooting
Dmax
Time at Dmax
Degradation Rate
Protein Recovery
Monitor protein: small molecule interactions
Monitor binding affinity and rank order compounds
Measure kinase inhibitor/degrader occupancy, selectivity, and affinity
Provide information of PROTAC cell permeability by both live cell and permeable assay mode
Monitor PPI in living cells
Monitor binding affinity and residence time
Rank order compounds
Endpoint analysis vs kinetic analysis
Ratiometric assay
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