BioDuro-Sundia offers a suite of assays for in vitro PROTAC evaluation
Target protein degradation
HiBiT cell line generation using CRISPR-Cas9 technology and evaluation of PROTAC compounds in HiBiT cells
Simple Western with better quantification, less variation, higher reproducibility, shorter turnaround time and lower protein consumption
Western-blot, In-Cell Western/In-Cell ELISA, ELISA, FACS, HTRF, etc.
Cell permeability and target engagement
NanoBRET Target Engagement assay
Binding to POI or E3
Live cell and permeable modes
HTRF, SPR, Co-IP, FP, TSA, etc.
Ternary complex formation
NanoBRET PPI assay
SPR, TR-FRET, AlphaLISA, Co-IP, etc.
Ubiquitination assays and proteasomal recruitment assays