Fluorescence, absorbance, luminescence, FP, TR-FRET, HTRF, LANCE, Alpha
Filter binding, SPA and other radiometric assays
LC/MS
SPR
FLIPR
HiBiT
Simple Western, Western-blot, In-Cell Western, ELISA
FACS and CBA multiplex
High content imaging
PBMC, T cell, B cell and NK cell isolation
T cell activation and proliferation assays
Immuno-phenotyping or biomarkers analysis with multi-color FACS, CBA, ELISA, RT-PCR
Cell proliferation, apoptosis, pyroptosis, migration and invasion, cell cycle analysis
Antibody-dependent cell-mediated cytotoxicity (ADCC)
ADC assays
BioDuro-Sundia offers a suite of assays for in vitro PROTAC evaluation
Target protein degradation
HiBiT cell line generation using CRISPR-Cas9 technology and evaluation of PROTAC compounds in HiBiT cells
Simple Western with better quantification, less variation, higher reproducibility, shorter turnaround time and lower protein consumption
Western-blot, In-Cell Western/In-Cell ELISA, ELISA, FACS, HTRF, etc.
Cell permeability and target engagement
NanoBRET Target Engagement assay
Binding to POI or E3
Live cell and permeable modes
HTRF, SPR, Co-IP, FP, TSA, etc.
Ternary complex formation
NanoBRET PPI assay
Endpoint
Kinetic
SPR, TR-FRET, AlphaLISA, Co-IP, etc.
Ubiquitination assays and proteasomal recruitment assays
Pseudo-virus entry assay
Main protease (3CL Mpro) FRET-based biochemical assay
Papain-like protease (PLpro) fluorescence assay
TR-FRET based ACE2/RBD binding assay
SPR based binding assay
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