NASH
Comprehensive studies that elucidate pharmacodynamics, mechanism of action, and efficacy of candidate compounds. In vivo and in vitro studies are conducted with utmost care and compliance with best industry practice.
Liver/Renal Disease
Liver/Renal Services
- Liver weight
- Liver Function: ALT, AST
- Lipid panel (liver & serum): TC, TG, FFA
- Insulin resistance: Insulin, BG
- Histology: HE
- Hepatic Fibrosis markers
- Collagen type 1a, 3a, α-1 Procollage
- SMA-a, TGF-b
- Hydroxyproline
- Histology Sirius Red staining
- Hepatic Inflammation markers
- TNF-a levels
- Mononuclear cell infiltration
- Oxidative Stress:
- Reactive oxygen species
- Hydroxynonenal (HNE) level
NASH
BioDuro-Sundia’s pharmacology team has developed highly clinically translatable animal NASH models to evaluate drug candidates. With short NASH induction periods of 12 – 16 weeks in mice and 8 – 12 weeks in hamsters our NASH models successfully mimic the pathogenesis of clinical NASH patients and exhibit hallmarks of NASH, including steatosis, lobular inflammation, ballooning and fibrosis.
- Clinical Relevance
- Obesogenic NASH models featuring all the major indicators of NAFLD to moderate NASH, including dyslipidemia, inflammation, ballooning and robust fibrosis.
- Pharmacologic responses
- Reference compounds (OCA, Elafibrinor, Semaglutide) in our high fat diet + modified high fat diet (HFD+mHFD) NASH mouse model match the responses of these experimental drugs in clinical trials
- Histology and biomarkers
- Demonstrate clinically relevant levels of disease phenotype & scores.
- Includes steatosis, ballooning, inflammation, mallory body and fibrosis
- Metabolic profiles
- Similarly altered obesity markers as does human NASH, including insulin, glucose, cholesterol, triglycerides and free fatty acid.
- Mechanisms of action
- Drugs targeting diverse biological pathways and processes
- Speed
- High fat diet + modified high fat diet (HFD + mHFD) mouse model with shorter induction period between 12 – 16 weeks
- High fat, high cholesterol + Fructose (HFC+F) hamster models with induction period between 8 – 12 weeks
- Comprehensive
- 5 different NASH disease models
- Multiple induction methods:
- Obesogenic (HFD, mHFD, AMLN)
- Chemical induction (CCl4, AMLN+CCl4)
- Nutrient deficient (0.1MCD)
- Collaborative & Responsive
- Group leaders & study directors provide rapid feedbacks and consultations to your preclinical model studies, including model selection, study design and data interpretation
NASH Models
- NASH Models
- NAFLD/NASH Models & in Vitro Assays
- Nutrient-deficient NASH Models
- SD rat or C57bl mice fed on MCD diet for 1-2 weeks
- SD rat or C57bl mice fed on 0.1MCD diet for 2-6 weeks
- Chemically induced NASH Models
- AMLN/HFD + CCl4 in C57bl mice, 12+4 weeks
- Featured with inflammation, steatohepatitis and fibrosis
- Obesogenic dietary NASH Models
- HFD + modified HFD/WD in C57bl mice, 12-16 weeks
- Genetic NASH Models
- ob/ob mice with AMLN diet, 8-12 weeks
- Hamster NASH Model
- Golden Syrian hamsters
- High fat & high cholesterol (HFC) plus fructose, 8-12 weeks
- Fatty Liver