FULLY HUMANIZED TRANSGENIC MICE
With the evolution of gene manipulation technology, the mouse antibody V genes can be replaced by human ones to create the humanized mice. Immunization of such mice to generate antibodies with human V region sequences has been widely adopted for therapeutic antibody discovery.
BioDuro-Sundia now has the humanized mice for human antibody generation. All the endogenous murine Ig segments have been deleted and replaced by human ones including V/D/J genes for VH and V/J genes for VL. The immunization response tests have shown that the humanized mice perform even slightly better than the normal mice. The expression of these Ig genes was analyzed by NGS and compared with their expression pattern in human. The results indicate that the expression levels are similar in humanized mice and in human. All the data support that the humanized mice is an ideal platform for human antibody generation. It can also be combined with our DNA or cell immunization method for better outcome