Advanced Immunization Strategies
Technologies optimized for best support of discovery & development of biologics products.
ADVANCED IMMUNIZATION STRATEGIES
BioDuro-Sundia has set up the next-generation animal-based antibody discovery platform for rapid and high-quality therapeutic antibody discovery with the integration of humanized mice, DNA/cell immunization and single B cell technologies. Aiming to establish a comprehensive and highly efficient antibody discover platform to meet the needs of our clients.
- Advanced immunization and fusion
The advanced immunization technologies such as immunizing animal with antigen coding DNA or surface antigen expressing cells which are preferred alternatives for targeting antigens that are difficult to express or purify. Usually, animal immunization can produce high affinity antibodies but the fusion efficiency by conventional method is low. At BioDuro we use electroporation for DNA immunization and fusion process to achieve better efficiency.
We have developed an immunization technology with multiple advantages, which is especially suitable for antigens such as membrane bound receptors, tumor associate antigens, immune regulators and GPCRs:
- Novel plasmid with enhancing elements regulating expression and immunogenicity, which could keep constant / high-level expression and help to break the tolerance induced by homology.
- Variable strategies for antigen expression in native conformation, including DNA-based self-expressing, recombinant expression on cell surface, and antigen overexpressing tumor cell lines. Multiple adjuvants at different stages in the whole immunization process, which help to induce strong immune response.
- Co-expression with co-receptor or chaperones, which helps to keep correct modification and conformation, especially for antigens in membrane complex or are heavily glycosylated.